quantigene® bdna signal amplification kit  (Panomics Inc)

Journal: Oncology Letters

Article Title: Detection and clinical significance of circulating tumor cells in patients with nasopharyngeal carcinoma

doi: 10.3892/ol.2019.10560

Figure Lengend Snippet: Sequences for the bDNA signal amplification probes.

Article Snippet: The capture probes sequences for the CK18, Twist and CD45 genes (listed in ) and the sequences for the bDNA signal amplification probes (listed in ) were purchased from Invitrogen (Thermo Fisher Scientific, Inc.). table ft1 table-wrap mode="anchored" t5 Table I. caption a7 Gene Sequences (5′-3′) CK18 AGAAAGGACAGGACTCAGGC GAGTGGTGAAGCTCATGCTG TCAGGTCCTCGATGATCTTG CAATCTGCAGAACGATGCGG AAGTCATCAGCAGCAAGACG CTGCAGTCGTGTGATATTGG Twist ACAATGACATCTAGGTCTCC CTGGTAGAGGAAGTCGATGT CAACTGTTCAGACTTCTATC CCTCTTGAGAATGCATGCAT TTTCAGTGGCTGATTGGCAC TTACCATGGGTCCTCAATAA CD45 TCGCAATTCTTATGCGACTC TGTCATGGAGACAGTCATGT GTATTTCCAGCTTCAACTTC CCATCAATATAGCTGGCATT TTGTGCAGCAATGTATTTCC TACTTGAACCATCAGGCATC N-cadherin TGCATAATGCGATTTCACCA ACATTGAGAAGAGGCTGTCC GCTTCAGGCTCAATTTTACT TTCACTGACTCCTCAGTTAA GCTTACTGAATTGTCTTGGG TGGAGTTTTCTGGCAAGTTG Open in a separate window CK18, cytokeratin 18; CD45, cluster of differentiation 45.

Techniques: Amplification, Sequencing

Journal: Emerging Microbes & Infections

Article Title: Establishment of a fluorescent in situ hybridization assay for imaging hepatitis B virus nucleic acids in cell culture models

doi: 10.1038/emi.2017.84

Figure Lengend Snippet: FISH of HBV RNA and DNA in a hepatoma cell line supporting HBV replication. HepG2-NTCP ( A ) and HepAD38 ( B – D ) cells, which are either maintained in doxycycline-supplemented medium ( C ) or doxycycline-free medium, were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After bDNA amplification, fluorophore-conjugated label probe was applied and images were acquired with a Deltavision epifluorescence microscope ( A – C ) and Nikon 3D-SIM microscope (Tokyo, Japan) ( D ). Scale bar, 4 μm.

Article Snippet: The ViewRNA technology uses the bDNA signal amplification scheme, which was initially developed by the Chiron Corporation to quantify the HIV and HCV viral loads., , , The bDNA scheme can also be used to quantify DNA molecules, such as HBV DNA, with a detection limit of 2000 copies.

Techniques: Hybridization, Amplification, Microscopy

Journal: Emerging Microbes & Infections

Article Title: Establishment of a fluorescent in situ hybridization assay for imaging hepatitis B virus nucleic acids in cell culture models

doi: 10.1038/emi.2017.84

Figure Lengend Snippet: STORM imaging of HBV DNA. ( A ) HepAD38 cells cultured in doxycycline-free medium were fixed and permeabilized followed by hybridization with Probe set 2. After bDNA amplification, fluorophore-conjugated label probe was applied and images were reconstructed from stochastic images as described in the Materials and Methods section. Cell nuclei were imaged after STORM imaging using conventional fluorescence microscopy and merged. Scale bar, 5 μm. The surface areas ( B ) and circularity ( C ) of detected puncta in epifluorescence, 3D-SIM and 3D-STORM platforms were compared.

Article Snippet: The ViewRNA technology uses the bDNA signal amplification scheme, which was initially developed by the Chiron Corporation to quantify the HIV and HCV viral loads., , , The bDNA scheme can also be used to quantify DNA molecules, such as HBV DNA, with a detection limit of 2000 copies.

Techniques: Imaging, Cell Culture, Hybridization, Amplification, Fluorescence, Microscopy

Journal: Emerging Microbes & Infections

Article Title: Establishment of a fluorescent in situ hybridization assay for imaging hepatitis B virus nucleic acids in cell culture models

doi: 10.1038/emi.2017.84

Figure Lengend Snippet: Detection of HBV RNA and DNA in HBV virus-infected HepG2-NTCP cells. HepG2-NTCP cells were either mock infected ( A ) or infected with 100 × concentrated HepAD38 cell supernatant ( B – D ). Seven days after infection, cells were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After bDNA amplification, fluorophore-conjugated label probe was applied and images were acquired using a Deltavision epifluorescence microscope with a × 100 oil-immersion objective. Image ( D ) was an enlarged area in the rectangle region of ( C ). Scale bar, 4 μm.

Article Snippet: The ViewRNA technology uses the bDNA signal amplification scheme, which was initially developed by the Chiron Corporation to quantify the HIV and HCV viral loads., , , The bDNA scheme can also be used to quantify DNA molecules, such as HBV DNA, with a detection limit of 2000 copies.

Techniques: Virus, Infection, Hybridization, Amplification, Microscopy